Journal article
A village in a dish model system for population-scale hiPSC studies
DR Neavin, AM Steinmann, N Farbehi, HS Chiu, MS Daniszewski, H Arora, Y Bermudez, C Moutinho, CL Chan, M Bax, M Tyebally, V Gnanasambandapillai, CE Lam, U Nguyen, D Hernández, GE Lidgerwood, RM Graham, AW Hewitt, A Pébay, NJ Palpant Show all
Nature Communications | NATURE PORTFOLIO | Published : 2023
Abstract
The mechanisms by which DNA alleles contribute to disease risk, drug response, and other human phenotypes are highly context-specific, varying across cell types and different conditions. Human induced pluripotent stem cells are uniquely suited to study these context-dependent effects but cell lines from hundreds or thousands of individuals are required. Village cultures, where multiple induced pluripotent stem lines are cultured and differentiated in a single dish, provide an elegant solution for scaling induced pluripotent stem experiments to the necessary sample sizes required for population-scale studies. Here, we show the utility of village models, demonstrating how cells can be assigned..
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Grants
Awarded by National Health and Medical Research Council
Funding Acknowledgements
We are grateful to the participants who provided samples to generate the human-induced pluripotent stem cells used in this study. We also want to thank Antonio Garcia for the support on figures. This research was supported by a National Health and Medical Research Council (NHMRC) Investigator grant (J.E.P., 1175781), an NHMRC Senior Research Fellowship (A.P., 1154389), by research grants from Stem Cells Australia - the Australian Research Council Special Research Initiative in Stem Cell Science, and Discovery Project (190100825), the Yulgilbar Alzheimer's Research Program(A.P. and J.E.P.), the NHMRC (1143163; 1181010), Goodridge Foundation, and the DHB Foundation (G.E.L. and A.P.).